Fig.: IHC of human IDH1 R132H in formalin-fixed paraffin-embedded brain tissue sections: The IDH1 R132H mutation specific monoclonal antibody clone H09 displays a strong and specific reaction at a 1:40 dilution. In contrast, at a 1:40 dilution antibody clone HMab-1 shows a very faint staining which remains weak even at lower dilutions of 1:20 and 1:10. All sections were stained by an indirect alkaline phosphatase method according to standard procedures with antigen retrieval by high-temperature heating in citrate buffer (pH 6.0) and counterstaining with Hematoxylin.
The exclusive production of antibody clone H09 by dianova together with its widespread commercial availability since 2010 has changed routine diagnostic neuropathology: It provides an unrevaled accuracy to the diagnosis of infiltrating gliomas (WHOII/III) and secondary gliomas. At present many companies non-exclusivly offer a different clone, HMab-1. By comparative IHC analysis we demonstrate that clone H09 displays an unchallenged staining quality at considerably higher dilution.
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